About 100 yards of Newell Drive will be limited to one lane of traffic starting this morning and lasting until Tuesday.
The work is part of the construction of an underground tunnel that will link the Shands at UF hospital to the Shands Cancer Hospital.
The area will span 100 yards of Newell Drive adjacent to the Shands at UF Emergency Department, according to a Thursday news release.
The tunnel, scheduled for completion in late November, will give hospital staff, patients and visitors a 440-foot path they can walk through instead of having to cross Southwest Archer Road.
The road will be converted to one-lane traffic because a storm water pipe is being installed, said Ted Kruljac, a Shands spokesman.
"We purposely scheduled the construction during an away-game weekend," he said.
Although traffic may become more crowded in that area, Kruljac said everyone will have the same access to the hospital. Visitors will be able to access the Shands east visitor parking garage, according to the news release.
Kruljac said that there are no plans to close part of Newell Drive again for construction.
